Composite

Part:BBa_K2638751:Design

Designed by: Antonin Lenzen   Group: iGEM18_Bielefeld-CeBiTec   (2018-10-10)


Synthetic Origin of Replication B


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 684
    Illegal NheI site found at 707
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This Ori was designed base on the modeling of Vilnius 2017 as well as on our own modeling and tests of promotor strength to maintain a plasmid copy number feasible for our measurement system.


Source

iGEM Parts Registry, 2018 Distribution Plates

References